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Imaging & Microscopy
Core
Objective
The goal of
the Neuroscience Institute's Imaging and Microscopy
Core is to provide centralized state-of-the-art microscopic
and imaging technologies as well as technical assistance
with histological tissue processing, immunofluorescence
staining, confocal microscopy, and image analysis. The
Microscopy and Imaging Core will support and facilitate
users by:
Maintaining a centralized,
cost-effective resource for high quality photo microscopy,
imaging and image analysis services. Continuing to provide
cutting-edge services and techniques to enhance experimental
design, data acquisition, data analysis and presentation.
Providing state-of-the-art
digital photographic and image output technologies to
ensure highly consistent image quality for techniques
performed in the respective user laboratories.
Providing training
in immunohistochemistry and digital image capture, processing
and analysis.
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Staffing of the
Core
Director:
Byron Ford, Ph.D
Core Director for the Microscopy and Imaging Core Facility.
Technician:
The Core has a dedicated technician who has experience and
expertise in histology and a number of other techniques.
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Resources and Environment:
All facilities offered by the Core are
located within the Neuroscience Institute on the second
floor of the Multidisciplinary Research Center. The Microscopy
and Imaging Core laboratory will provide investigators
access to the following technologies (including training,
information transfer, quality control and consultations):
Microscopy technologies:
- Light microscopy (including bright-field, phase-contrast,
dark field, and intensified low-light)
- Single and dual chromophore indirect immunofluorescence
- Photo microscopy on 35 mm film
- Confocal and color digital photo microscopy
Digital image analysis:
- Quantitative analysis using microscopy technologies
listed above
- Morphometry
- Object identification, counting and gray level analysis
- Image processing (mathematical operations, pseudo
color generation, etc.)
- Spatial intensity quantitation of single and dual-emission
chromophores.
Digital photographic/imaging services:
- Color image and presentation recording on 35mm film
from PowerPoint, tiff images, etc.
- High resolution digital scanning of 35 mm film (positives
and negatives)
- High resolution digital flat-bed scanning of transparent
and opaque films, x-rays, polyacrylamide gels, westerns
and photographs.
- Adobe Superscript PhotoShop preparation of digital
images
- Image file archiving and network file server access
Microscopy equipment available
in the Core include the following:
- Zeiss Axioscope (upright) Microscope with epifluorescence
photo microscopy (100W Hg pwr source) attached to a 23GB
HD
- Dell Precision 420 computer running on a Windows
NT workstation.
- Olympus IX70 (inverted) Microscope with epifluorescence,
brightfield, phase contrast and Nomarski DIC (100W Hg
bulb burner) attached to a 4.0GB HD Windows NT workstation.
- Olympus SC35 and Zeiss photo micrographic camera systems.
- Olympus Fluoview Control Unit and Photomultiplyer
tube unit.
- Anti-vibration Table.
- NEC Argon Gas LASER with power supply.
- Omnichrome Series 43 Krypton LASER with Ion laser
power supply.
- Optronics CCD camera
- SPOT Cooled CCD 36-bit color camera with adapter for
both microscopes
- A wide selection of high quality objectives for phase,
DIC, bright field and fluorescence microscopy between
4x-60x magnification.
Image analysis capabilities include:
- Image Pro image analysis program<
- Diagnostic Imaging basic 3.2.4 SPOT software
- Diagnostic Imaging Advanced 3.2.4 SPOT software
- Fluoview 2.0 confocal image analysis program
Image scanning/output capabilities
- HP 4C flatbed color scanner (600 dpi) with transparency
adapter
- Polaroid Sprintscan 35 mm color scanner (2700 dpi)
with PathScan enabler
- Kodak 1650 PS Dye Transfer color photographic quality
network printer
- Agfa PCRII color 4000 line 35 mm film scanner/recorder
- Three Pentium-based workstations, with recordable
CD-ROM, ZIP and JAZ drive capabilities.
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