Technician:
The Core will have a dedicated
technician who has experience and expertise in gene expression
profile technology, mutagenesis of clone DNA, introduction
of DNA into mammalian cells, preparation of
primary neuronal and non-neuronal cell cultures and a
number of other recombinant DNA techniques.
Resources and Environment:
All facilities offered by the Core are located
in the Neuroscience Institute.
Primary Equipment of the
Cell and Molecular Core Facility:
Gene expression profile analysis: Affymetrix:
Hardware: Hybridization oven, Fluidics station
400, Gene Array (Neuroscience Institute is in the
final steps in acquiring the scanner), and PC-compatible
workstation.
Software: Microarray software solutions,
and Data Mining Tool
Real Time PCR Detection System: Biorad iCycler
Multi-Color
Hardware: iCycler IQ System, Dell OptiPlex
GX260 Computer
Software: iCycler iQ Real Time Detection
System Software
Molecular Signaling Analysis: Berthold Microplate
Luminometer
Hardware: Microplate Luminometer, Dual Injector
Unit
Software: Simplicity (Version 2.0 R1)
Gene Delivery System: Helios gene gun (Bio-Rad)
Hardware: Gene gun, tubing preparation station,
tubing cutter, helium regulator and hose assembly, and
optimization kit
Accessory Equipment for Cell
and Molecular Core Facility:
Two Vertical Flow Biological Safety Cabinet (4
and 6 foot, Baker Sterilgard III Advance, Class II, Type
A/B3)
Three Single Chamber Water Jacket Incubators (Forma,
model #3110)
Two Benchtop Incubator Orbital Shakers (Forma, model
#420)
Two EdgeGARD Hoods (Baker)
Refrigerated Centrifudge (Eppendorf)
Spectrophotometer (Beckman, model # DU650)
Versadoc (Bio-Rad)
Microplate Reader (Bio-Rad)
PhosphoImager (Bio-Rad)
Administration:
Dr. Jackson will be responsible for the overall administration
of the Cell and Molecular Core. He will guide
the day-today operations and activities of the Core, make
key decisions of priority, and evaluate new
technologies for adaptation.
