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The Image Analysis Facility houses five major pieces of equipment:
- Molecular Dynamics Confocal Laser Scanning Microscope
- Video-based digital imaging microscope
- Tecan Multiwell Plate Reader
- Millipore Cytofluor Multiwell Fluorescence Plate Reader
- Zeiss Axioplan epifluorescent microscope with a Digital
Camera.
The facility provides a wide variety of options for the analysis
and quantification of fluorescent probes, markers and antibodies
within fixed and living cells.
All of the computers within the facility are now networked
to each other as well as to the institutional network. Included
in the network is the Codonics printer. The tremendous
advantage of the networked environment in the facility is that
images can be acquired on the confocal workstation and then
rapidly and easily transmitted either to the other workstation
for analysis or to the Codonics printer for the generation
of hard-copy, publication-quality images. In terms of
productivity, users no longer have to wait until the workstation
on the confocal is available for data analysis. In addition,
several investigators are now printing publication-quality images
from their office or lab PC’s by remote access of the Codonics
printer facilitating their productivity as well as increasing
the use of the laboratory by the biomedical research faculty.
Also, a Zeiss Axiovert 25 inverted cell culture microscope
was purchased in the past year. The acquisition of this
microscope alleviates the necessity of carrying one’s cells
down the corridor to the departmental cell culture microscope
thus facilitating the ease with which investigators can prepare
and examine the cells used in the facility.
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